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    您现在的位置:yy智勇三张牌 > 技术服务 > 分子生物学服务 > 基因表达检测WB >
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    幸运三张牌:Western blot检测


    Western Blot
        免疫印迹immunoblotting又称蛋白质印迹western blot,它是根据抗原抗体的特异性结合检测复杂样品中的某种蛋白的方法。Western blot是在凝
    胶电泳和固相免疫测定技术基础上发展起来的一种新的免疫生化技术。由于western blot具有SDS-PAGE的高分辨力和固相免疫测定的高特异性和敏
    感性,现已成为蛋白分析的一种常规技术。Western blot常用于鉴定某种蛋白,并能对蛋白进行定性和半定量分析。 
        权阳生物为提供标准western blot技术服务(您需自备一抗)。并对band进行灰度分析,得出蛋白表达相对定量结果。
    服务要求
        需新鲜或正确保存的蛋白样品或者蛋白粗提液,阳性对照样品(如果有), 检测目的蛋白的一抗(或由Transheep代购)。组织样品不少于50mg
    ;细胞不少于5×106个;总蛋白浓度不低于 2μg/μl(至少50μl)。
    服务基本流程
        1. 蛋白提取和定量(如果提供蛋白样品,则直接电泳预实验检测抗体特异性);
        2. SDS-PAGE 10-15% 变性聚丙烯酰胺凝胶电泳;
        3. 转膜(PVDF膜);
        4. 封闭 (5%脱脂奶粉);
        5. 一抗孵育(其中内参基因为Tubulin或HistoneH3.1);
        6. 二抗孵育(如果由于材料降解或一抗造成实验无法继续,则收相关预实验费用);
        7. 显色(ECL);
        8. 曝光、洗片、灰度分析;

    服务流程


    服务说明


    Western Blot基因表达范例
    收集生长状态良好的目的细胞,提取蛋白,采用Western Blot的方法检测目的基因的表达情况。
    检测结果如下:   


     PC为抗体说明书建议的作为阳性对照用细胞蛋白样品。上图显示A细胞目的基因表达丰度更高。Western blot检测抗体是否有效,
    检测目的细胞中目的基因的内源表达。 

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    Transheep 有一套成熟的质检程序,产品发货前均经过质量检测,确保您收到的产品质量无误! Transheep拥有大量的基因现货产品,本公司的现货产品从订货日期起一周以内即可到货,大大缩短了您的等待时间!另外Transheep的一站式克隆平台也能让您体验到前所未有的快速定制服务! Transheep所售出的产品全部登记在册,如果您拿到我们的产品觉得和实质不相符或存在问题,您只需要告诉我们相关产品信息我们会迅速做出反应,为您提供解决方案或退款!
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    联系我们→客户或销售人员与您沟通→签订合同→支付预付款→执行项目→确认收货→支付尾款→售后服务 Transheep提供免费的技术咨询,任何关于产品或者技术问题,都可以联系我们们。 免费热线:400-0179890 服务邮箱:[email protected] 基因合成
    亚克隆构建
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  • 【专题】河北省无偿献血领导小组会议报道 2019-05-05
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