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  • 欢迎来到上海权阳生物科技有限公司官网
    您现在的位置:yy智勇三张牌 > RNAi产品 > shRNA载体构建 > shRNA慢病毒包装 >
    产品
    请输入基因编号或名称:
    产品分类:
                                                                                                       shRNA慢病毒包装
    服务介绍

    慢病毒(Lentivirus) 载体是以HIV-1 (人类免疫缺陷Ⅰ型病毒)为基础发展起来的基因治疗载体。区别一般的逆转录病毒载体,它对分裂细胞和非分裂细胞均具有感染能力。

    慢病毒载体可以将外源基因或外源的shRNA有效地整合到宿主染色体上,从而达到持久性表达目的序列的效果。在感染能力方面可有效地感染神经元细 胞、肝细胞、心肌细胞、肿瘤细胞、内皮细胞、干细胞等多种类型的细胞,从而达到良好的的基因治疗效果。对于一些较难转染的细胞, 如原代细胞、干细胞、不分化的细胞等,使用慢病毒载体,能大大提高目的基因或目的shRNA的转导效率,且目的基因或目的shRNA整合到宿主细胞基因组 的儿率大大增加,能够比较方便快捷地实现目的基因或目的shRNA的长期、稳定表达。

    所以,在体外实验及体内实验的研究中,慢病毒己经成为表达外源基因或外源shRNA的常用载体形式之一, 并且正在获得越来越广泛的应用。

    服务内容

    - ▶yy智勇三张牌计划软件▶yy智勇三张牌天天计划▶yy智勇三张牌人工计划▶yy智勇三张牌在线计划▶yy智勇三张牌稳赢计划
    实验方案 服务描述 滴度 价格 服务周期
    1 客户提供靶点序列,我们构建到指定载体,包装慢病毒 1x108 TU 2500元/靶点病毒 2-3周
    2 客户根据目的基因从我们shRNA慢病毒库中选择shRNA现货质粒,我们包装成慢病毒 1x108 TU 2500元/靶点病毒 2 - 3 周
    3 客户提供基因名称,我们负责设计,提供客户3个靶点慢病毒,保证干扰效率在RNA水平70%以上 1x108 TU 8800元/套病毒 3 - 4 周
    4 客户根据目的基因从我们的shRNA慢病毒文库中选择质粒,我们提供质??寺?,客户自行验证效率后挑选有效靶点包装慢病毒 1x108 TU 1200元/套质粒 1周

    载体信息(可在我公司载体库中查看详情)

    载体名称 抗性 报告基因
    plko.1-TRC Puro /
    plko.1 puro Puro /
    plko.3G Puro /
    pLVX-sh1 Puro /
    pLKO-puro-TurboGFP Puro TurboGFP
    pLVX-sh2 / EGFP
    pLVX-U6-RFP-Puro Puro RFP
    pLVX-U6-GFP-Puro / GFP
    pLVX-U6-EGFP Puro EGFP
    pLVX-U6-tdTomato Puro tdTomato
    pLVX-U6-YFP Puro YFP
    pLVX-U6-Neo Neo /
    pLVX-U6-Neo-EGFP Neo EGFP
    pLVX-U6-Puro-Tet-on Puro  

    备注:本公司慢病毒系统包装质粒为PSPAX2 及PMD2G

    订购流程

    1.质量保证 2.发货时间 3.关于售后
    Transheep 有一套成熟的质检程序,产品发货前均经过质量检测,确保您收到的产品质量无误! Transheep拥有大量的基因现货产品,本公司的现货产品从订货日期起一周以内即可到货,大大缩短了您的等待时间!另外Transheep的一站式克隆平台也能让您体验到前所未有的快速定制服务! Transheep所售出的产品全部登记在册,如果您拿到我们的产品觉得和实质不相符或存在问题,您只需要告诉我们相关产品信息我们会迅速做出反应,为您提供解决方案或退款!
    4.订购流程 5.联系我们 6.相关服务:
    联系我们→客户或销售人员与您沟通→签订合同→支付预付款→执行项目→确认收货→支付尾款→售后服务 Transheep提供免费的技术咨询,任何关于产品或者技术问题,都可以联系我们们。 免费热线:400-0179890 服务邮箱:[email protected] 基因合成
    亚克隆构建
    3,UTR验证实验
    启动子合成及验证
    慢病毒包装
    腺病毒包装
    稳转株构建
  • 【专题】河北省无偿献血领导小组会议报道 2019-05-05
  • 我写文章不是为了别人的赞许,是为了讨论问题,让人有思考的价值,就像你网名一样,探寻真理。我并非就全盘赞成市场经济,只是在讨论它的合理性,在文中也提问,“既然我们 2019-05-05
  • 机构改革是一场政治考验 2019-05-03
  • 西安“千人计划”专家创业 最高可获450万个人奖励 2019-04-27
  • 学习有理丨着力改善环境 增创发展优势 2019-04-23
  • 危急时刻,她把生的希望留给学生 2019-04-19
  • 工人日报:与百姓交流少用些拗口术语 2019-04-15
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  • 口音让他露马脚 背负3条人命公安部B级逃犯被武汉细心民警揪出 2019-02-27
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