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  • 欢迎来到上海权阳生物科技有限公司官网
    您现在的位置:yy智勇三张牌 > RNAi产品 > shRNA载体构建 >
    产品
    请输入基因编号或名称:
    产品分类:
                                                                                                                  shRNA载体构建

    服务介绍

    shRNA(short hairpin RNA, shRNA)是一段小发卡或短发卡RNA序列,一般长19~23bp,常被用于RNA干扰沉默靶基因的表达。利用载体把shRNA导入细胞,载体中的U6或H1启动子确保shRNA的表达;shRNA的发卡结构可被细胞机制切割成siRNA,然后siRNA结合到RNA诱导沉默复合物上(RNA induce
    -d silencing complex,RISC),该复合物能够结合到目的mRNAs并将其降解。通过载体可以将shRNA整合到细胞基因组中,从而使细胞长期稳定表达shRNA。
    权阳生物可提供多种shRNA表达载体,如慢病毒载体、腺病毒载体、腺相关病毒载体、以及普通的真核表达载体等;同时也有多种荧光报告基因可供选择,如GFP、eGFP、tdTomato、YEP等;另外还有药筛基因可供选择:Puromycin、Neomycin等,用于后期构建稳定表达shRNA的细胞株。

    服务流程

    1、接受客户目标基因信息; 
    2、分析目标基因,并设计高质量的shRNA序列(客户可从Transheep shRNA 文库中自行选择序列); 
    3、PCR退火形成shRNA双链; 
    4、将shRNA双链克隆至含有U6或H1启动子的表达载体上; 
    5、测序以确保插入序列的正确性; 
    6、向客户提交测序结果与实验方法报告单。

    载体选择(可到Transheep 质???/span>中选择相应载体)

    载体举例
    <
    慢病毒载体   腺病毒载体
    plko.1-TRC   pShuttle-U6
    plko.1 puro   pShuttle-U6-CMV-RFP
    plko.3G   pShuttle-U6-CMV-EGFP
    pLVX-sh1    
    pLKO-puro-TurboGFP    
    pLVX-sh2    
    pLVX-U6-RFP-Puro    
    pLVX-U6-GFP-Puro    
    pLVX-U6-GFP    
    pLVX-U6-tdTomato    
    pLVX-U6-YFP    
  • 【专题】河北省无偿献血领导小组会议报道 2019-05-05
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